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Whittle, D.J., Kilburn, D.G., Warren, R.A.J. and Miller, J.R.C. (1982) Molecular Cloning of a Cellulomonas fimi Cellulase Gene in Escherichia coli: Recombinant DNA; Plasmid pBR322; Immunoassay. Gene, 17, 139-145. https://doi.org/10.1016/0378-1119(82)90066-X

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• TITLE: Study on Cellulase Gene Expressed in Pichia pastoris and Analyses of Its Biochemical Characters

  AUTHORS: Min Lu, Juan Chang, Ping Wang, Qingqiang Yin, Xiaowei Dang, Tianzeng Gao, Fushan Lu

  KEYWORDS: Cellulase Gene, Cloning and Expression, Biochemical Characters, Pichia pastoris

  JOURNAL NAME: Journal of Materials Science and Chemical Engineering, Vol.6 No.7, July 4, 2018

  ABSTRACT: Objectives: In order to increase cellulose degradation, cellulase was expressed in this study. Literature Review: Cellulose is the most abundant organic carbon source on Earth; its enzymatic hydrolysis will be very useful for bioenergy production and resource recycling. Methods: Cellobiohydrlase I (CBH I) gene was amplified from genomic DNA of Trichoderma koningii and inserted into pGAPZα A plasmid to construct the vector of pGAPZαA-CBH I. It was linearized and transformed into Pichia pastoris by electroporation. The recombinant Pichia pastoris was selected and incubated with YPD medium for cellulase secretion. Results: The result showed that CMCase and avicelase activity in the supernatant was 1.1798 U/mL and 0.1276 U/mL, the molecular weight of the expressed protein was 53 kDa determined with SDS-PAGE analyses, and the optimal temperature and pH of the expressed cellulase were 45?C - 50?C and 4.5 - 5.0, respectively. Conclusion: Cellulase gene from T. koningii has been successfully cloned and expressed in Pichia pastoris.