TITLE:
Inactivated Vaccine Trial of Mycoplasma gallisepticum in Ethiopia
AUTHORS:
Legesse Bekele, Temesgen Assefa
KEYWORDS:
Adjuvant, Chickens, Inactivated Vaccine, Mycoplasma gallisepticum, PCR
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.8 No.6,
June
29,
2018
ABSTRACT: The study and entire laboratory
works were conducted from December 2014 to April 2015 in National Veterinary Institute,
Bishoftu, Ethiopia. Formaldehyde
inactivated Montanide ISA70 based Mycoplasma
gallisepticum (MG) trial vaccine strain was confirmed the identity with
known primer using PCR from locally isolates of National Veterinary Institute
of Ethiopia. This study was aimed to develop
formaldehyde inactivated Montanide ISA70 based MG vaccine in
Ethiopia. It can help to device strategies in controlling the disease mainly
through developing more effective vaccine which will replace the currently
being imported vaccines by some farms. After culturing procedure, oil based
inactivated MG trial vaccine was produced in suitable clean and secure
accommodation. In this study, among different isolates, local isolate of Samuel
farm in NVI was prepared and evaluated in chickens. The amount of immune
antigen per 0.5 ml of
the dose was 107 Colony forming units (CFU) of the bacteria. The
trail vaccine was prepared and evaluated at the age of 16 weeks of chickens; the chickens were randomly divided into three
groups (A, B and C), each having twenty birds (10 male and 10 female). Each of
group B was vaccinated group of imported-live vaccine with 30 μl intraocularly for comparing with
inactivated trial vaccine, each bird of group C was inoculated with 0.5 ml
indigenous or trial vaccine subcutaneously at mid neck region and group A was
used as a control then challenge tests were performed. After challenge test,
among non-vaccinated chickens (control or group A) 2 chickens were died (10%),
thicken and cloudy appearance of the air sac showed 18 (90%), 2 chickens were
not showed thickened and cloudy air sack (10%). Although among vaccinated group
(inactivated vaccine or group C), all chickens did not show clinical signs or
post mortem changes (100%). From attenuated imported live vaccine (group B), no
clinical signs or post mortem changes were observed (100%). It was concluded that oil based MG
vaccine induces protective level of anti MG antibodies in chickens.