TITLE:
Restriction fragment length polymorphism in the exon 2 of the BoLA-DRB3 gene in Chinese Holstein of the south China
AUTHORS:
Xiu-Xiang Wu, Zhang-Ping Yang, Xiao-Long Wang, Yong-Jiang Mao, Shu-Chun Li, Xue-Kui Shi, Ying Chen
KEYWORDS:
PCR-RFLP; BoLA-DRB3.2; Chinese Holstein; Genetic Polymorphism; SCC
JOURNAL NAME:
Journal of Biomedical Science and Engineering,
Vol.3 No.2,
March
2,
2010
ABSTRACT: The genetic diversity of the exon2 of BoLA-DRB3 (BoLA-DRB3.2) in Chinese Holstein cattle of the south China was investigated by hemi-nested polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Six, four and eleven RFLP patterns were found after digestion with the restriction enzymes Hae III, Bst YI and Rsa I, respectively. The DNA sequence showed and twenty- five DRB3.2 alleles. GLM model analysis indicated that lactation and calving season have positive correlation with SCC (Somatic Cell Count) (p