TITLE:
Effects of Endoplasmic Reticulum Stress on Pulmonary Hypertension in Rat Induced by Chronic Hypoxia and Hypercapnia
AUTHORS:
Congcong Zhang, Jingjing Zhang, Yuanling Wu, Yongyue Dai, Lei Ying, Wantie Wang
KEYWORDS:
ERS, Pulmonary Artery Hypertension, Hypoxia Hypercapnia, Rat
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.6 No.6,
June
22,
2018
ABSTRACT: Objective: To observe the
pulmonary vascular remodeling in rats with pulmonary hypertension induced by
hypoxia and hypercapnia, and to explore the role of endoplasmic reticulum
stress in pulmonary hypertension. Methods: 1) 40 SD rats were randomly
divided into four groups: normoxic control group (N), hypoxia hypercapnia group
(HH), endoplasmic reticulum stress (ERS) inhibitor 4-phenyl butyric acid group
(4-PBA), ERS pathway agonist tunicamycin group (TM). 2) The mean pulmonary
arterial pressure (mPAP) and the right ventricular hypertrophy index (RV/(LV + S)) were
measured in each group. 3) Identification of pulmonary artery smooth muscle
cells (PASMCs) in each group by immunofluorescence α-SMA. 4) Morphological changes of lung tissue and pulmonary artery
were observed by electron microscope. 5) The apoptotic index of PASMCs in each
group was detected by TUNEL. 6) Reverse transcription polymerase chain reaction
(RT-PCR) and Western Blot (WB) were used to detect the expression of ERS related protein and mRNA (GRP78, CHOP, JNK,
Caspase-12) in each group. Results: 1) Compared with the N
group, the mPAP, RV/(LV + S) and vascular wall area (WA)/total area (TA) value of HH group, 4-PBA group and TM
group were increased (P P P P P P P P P P Conclusion: Hypoxia and hypercapnia induced
pulmonary vascular remodeling may be related to the proliferation of PASMCs,
and ERS related factors (JNK, Caspase12 and CHOP) are involved in the
regulation of hypoxic hypercapnia.