TITLE:
Determination of Azacitidine by Spectrophotometric Method
AUTHORS:
B. Ramachandra, N. Venkatasubba Naidu
KEYWORDS:
UV-Visible Spectrophotometry, Azacitidine, Blood Sample, MBTH/FeCl3 and Oxidative Coupling Reaction
JOURNAL NAME:
Open Journal of Medicinal Chemistry,
Vol.8 No.2,
June
6,
2018
ABSTRACT: Simple, selective and sensitive spectrophotometric method has been developed for the determination of Azacitidine in pharmaceutical formulations and blood with MBTH (3-methyl-2-benzothiazolone hydrazone hydrochloride) reagent, at PH-4.0 which is extractable at 620 nm. Beer’s law is obeying in the concentration ranges 10 - 35 μg·ml-1 for formulations and 4 - 24 μg·ml-1 for blood sample. %R.S.D was found to be 0.0240%, 0.0610 and Recovery 99.82% 99.24% respectively. The method was completely validated and proven to be rugged. The interferences of the other ingredients and excipients were not observed. The repeatability and the performance of the proven method were conventional by point and interior proposition and through recovery studies.