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Yoshikawa, H., Matsubara, K., Qian, G.S., Jackson, P., Groopman, J.D., Manning, J.E., Harris, C.C. and Herman, J.G. (2001) SOCS-1, A Negative Regulator of the JAK/STAT Pathway, Is Silenced by Methylation in Human Hepatocellular Carcinoma and Shows Growth-Suppression Activity. Nature Genetics, 28, 29-35.
https://doi.org/10.1038/88225

has been cited by the following article:

• TITLE: Methylation and Expression Status of SOCS1 and SOCS3 in Oral Lichen Planus

  AUTHORS: Kurara Yoshimura, Naomi Yada, Kou Matsuo, Hisako Hikiji, Daigo Yoshiga, Manabu Habu, Masaaki Sasaguri, Kazuhiro Tominaga

  KEYWORDS: Oral Lichen Planus, SOCS3, SOCS1, Methylation

  JOURNAL NAME: Open Journal of Stomatology, Vol.8 No.5, May 21, 2018

  ABSTRACT: Oral lichen planus (OLP) is a disease of unknown etiology affecting oral mucosa by mediated chronic inflammation and is classified as a potentially malignant oral disorder. SOCS1 and SOCS3 in the SOCS family have been identified as negative regulators of the cytokine-activated JAK/STAT pathway responsible for inflammatory reaction. The DNA methylation in the promoter regions of SOCS1 and SOCS3 have been reported to correlate with carcinogenesis. In this study, we performed methylation-specific PCR (MSP) to investigate the methylation status of the promoter regions in SOCS1 and SOCS3 genes using biopsy samples from OLP and oral squamous cell carcinoma (OSCC) patients. SOCS1 was methylated in 14/29 (48.3%) cases with OLP and 7/15 (46.7%) cases with OSCC. At the same time, SOCS3 was methylated in 25/29 (86.2%) cases with OLP and 11/15 (73.3%) cases with OSCC. We didn’t recognize any DNA methylation in SOCS1 or SOCS3 genes from the exfoliated cytological specimens of normal buccal mucosa. Furthermore, mRNA expression level was analyzed using real-time RT-PCR method to evaluate the correlation with DNA methylation status. DNA methylation status of SOCS1 seemed not to affect the expression level of SOCS1 mRNA. At the same time, DNA methylation status of SOCS3 was negatively correlated with the expression level of SOCS3 mRNA (p