TITLE:
Expression Analysis of MiR-124 in Experimental Cerebral Hypoperfusion
AUTHORS:
Tang Li, Huizi Lei, Linhui Wang, Yu Li
KEYWORDS:
MiRNA-124, Cerebral Hypoperfusion, 2VO, OGD
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.6 No.5,
May
21,
2018
ABSTRACT: MicroRNAs (miRNAs) are small noncoding single-stranded RNA molecules that act as negative regulators of gene expression and modulate the stability and/or the translational efficiency of target messenger RNAs. Studies have shown that miRNAs control diverse aspects of brain disease. In this study, the expression of miR-124 was investigated to explore the possible impacts of them on cerebral hypoperfusion. The model of aging rats with cerebral hypoperfusion was established by permanent occlusion of bilateral common carotid arteries (2VO). The expression of miR-124 was determined by real-time PCR. Cell cycle analysis was performed by fluorescence-activated cell sorting (FACS). Results showed that compared with control group, the expression of miR-124 decreased at early stage after operation in 2VO rats, the lowest level appeared on day 7 (p