TITLE:
In Vitro Culture of Shell Gland Epithelial Cells in Japanese Quail (Coturnix coturnix japonica)
AUTHORS:
Osamu Kai, Kaoru Mineo
KEYWORDS:
Japanese Quail, Shell Gland Epithelial Cells, In Vitro Culture, Protoporphyrin, Fluorescence Microplate Reader
JOURNAL NAME:
Open Journal of Animal Sciences,
Vol.8 No.2,
April
24,
2018
ABSTRACT: To investigate the secretion of protoporphyrin IX
(PpIX), superficial eggshell pigment, from shell gland cells of Japanese quail,
the epithelial cells of the gland were collected and isolated for cultivation in
vitro. An analysis of a peak for PpIX in the cells was performed using a
fluorescence microplate reader. The measurement showed that PpIX has a peak of
excitation wavelength at 410 nm and emission wavelength at 606 nm in the
culture medium (HamF12 + 4% HCl). Volumes of PpIX in the medium after 4 hour
culture of the cells were measured with a microplate reader using filter set of
excitation wavelength 400/30nm and emission wavelength 620/40nm. However the
cells did not secrete significantly PpIX during 4 hour incubation in this
culture system, addition of quail plasma to the medium resulted in significantly
higher secretion. A cultivation system in this study is able to use for the
study on the mechanism of the secretion of eggshell pigment, PpIX from Japanese
quail shell gland epithelial cells.