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Kaori, M., Petr, T., Miho, M.K., Tomohiko, K., Shusei, S., Danuse, T., Satoshi T., Goran, S. and Tatsuo, K. (2006) Roles of Arabidopsis ATP/ADP Isopentenyltransferases and tRNA Isopentenyltransferases in Cytokinin Biosynthesis. Proceedings of the National Academy of Sciences of the United States of America, 103, 16598-16603.
https://doi.org/10.1073/pnas.0603522103

has been cited by the following article:

• TITLE: Isolation and Functional Analysis of the Regulation of Branching by Isopentenyl Transferase Gene CmIPT1 in Chrysanthemum morifolium cv. ‘Jinba’

  AUTHORS: Abdurazak Ishak, Lili Dong, Huang Rong, Shuiming Zhang, Liangjun Zhao

  KEYWORDS: Chrysanthemum, Cytokinin, CmIPT1, Shoot Branching

  JOURNAL NAME: American Journal of Molecular Biology, Vol.8 No.2, April 13, 2018

  ABSTRACT: Today’s chrysanthemums are highly evolved flowering plants and they are considered as one of the most important ornamental cut flowers. In this research an isopentenyl transferase gene named CmIPT1 was isolated from Chrysanthemum morifolium cv. ‘Jinba’ using RACE and RT-PCR methods. The full cDNA sequence of CmIPT1 was 873 bp which encoded a deduced protein of 290 amino acids. It contained GxxGxGKS which is a conserved sequence of the typical domain of IPT family. The phylogenetic tree analysis of CmIPT1 in Chrysanthemum morifolium cv. ‘Jinba’ shows that it has the closest relationship with CcIPT1 in Cynara cardunculus var. scolymus. Expression of CmIPT1 was higher in stems and apex, whereas it was lower in leaves and roots. And the overexpression of CmIPT1 obviously increased the number of rosette branches in Arabidopsis. Here, in our study, we showed that CmIPT1 is a positive regulator of branch development in Chrysanthemum and may play a key role in regulating lateral branch formation of Chrysanthemum plants.