TITLE:
Insights on Blood Cytokines Production under Different In Vitro Mycobacterial Antigens in Tuberculosis Intestinal Parasites Co Infected Patients
AUTHORS:
Renan Jeremias da Silva, Fernanda C. Q. Mello, Janaína A. Leung, Antonio Henrique Almeida de Moraes Neto, Leila de Souza Fonseca, Helio Ribeiro de Siqueira, Health Care Victor Vala and CSEGSF Team, Maria Helena Féres Saad
KEYWORDS:
Mycobacterium tuberculosis, Intestinal Parasites, Mycobacterial Antigens, IgE, Cytokines
JOURNAL NAME:
Advances in Microbiology,
Vol.8 No.3,
March
21,
2018
ABSTRACT: Background: The concomitant presence of intestinal parasite infections (IP) and tuberculosis (TB) has relevance. M. tuberculosis immune response is associated with type 1 T helper cell (Th1) while IP is associated with type Th2 cell. However, there are several contradictory reports on cytokine production under coinfection and this could be in association to the mycobacterial antigens used in the studies. Aim: To get insight into the effects of different M. tuberculosis-specific antigens (ESAT-6/CFP-10 and 38 kDa/CFP-10) in generating of appropriate cytokines on peripheral blood mononuclear cells of IPTB co infected patients. Method: ELISA assessed IFN-γ and other 16 cytokines production and plasm IgE. In 18 months, we documented demographic, economic, clinical characteristics and IP frequency in individuals from Brazil. Results: An overall 10/35 (28.5%) were IPTB co infected and 40/76 (52.6%; p = 0.024) asymptomatic intestinal parasite infected community controls (IPCC). Endo-limax nana (40%) and Entamoeba coli (22%), were the most nonpathogenic protozoan identified and Entamoeba histolytica, Giardia intestinalis, Ascaris lumbricoides and Strongyloides stercoralis were the pathogenic species (40%). IgE was higher in IPCC (p = 0.036). Cytokine profiles were significantly biased toward a Th2 type IL-5 (p = 0.001) and IL-13 (p = 0.033), pro-inflammatory GM-CSF (p = 0.019) and borderline lower IL-1β in IPTB, all associated with ESAT-6/CFP-10, while IL-7 was borderline lower, but 38 kDa/CFP-10 associated; as well as IL-8 higher (p