TITLE:
Comparative Proteomics Reveals Set of Oxidative Stress and Thaumatin-Like Proteins Associated with Resistance to Late Blight of Tomato
AUTHORS:
Bruno Soares Laurindo, Renata Dias Freitas Laurindo, Patrícia Pereira Fontes, Camilo Elber Vital, Fábio Teixeira Delazari, Maria Cristina Baracat-Pereira, Derly José Henriques da Silva
KEYWORDS:
Solanum lycopersicum L., Pathogenesis-Related (PR) Proteins, Resistance to Phytophthora infestans
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.4,
March
20,
2018
ABSTRACT: Proteomics
techniques were used to study the molecular mechanisms involved in the defense
of tomato against late blight (Phytophthora infestans).
Proteins were extracted from resistant access BGH-2127 and susceptible cultivar “Santa Clara”. Leaves of the inoculated and non-inoculated (control) genotypes were
collected at 0, 2, and 48 h after inoculation and analyzed by two-dimensional
electrophoresis (2-DE), followed by identification with mass spectrometry
(MALDI TOF-TOF). A total of 56 differentially abundant proteins were
identified, of which 39 were resistant genotypes and 17 were susceptible.
These proteins were categorized into functional groups of energy and
metabolism, photosynthesis, stress and defense, transcription, other proteins,
and as un-characterized ones. For access BGH-2127, oxidative
stress proteins (2-cis peroxiedoxin BAS1 and 2-cis peroxiredoxin) and
thaumatin-like protein showed increase in the relative abundance at 0 and 48 h
of inoculation, respectively, and were therefore considered important for the
defense mechanism of this genotype. The expression standards evaluated by
real-time PCR differed from the results of the proteomic analysis. The
protein-protein interaction networks provided important information on the
cellular activities involved in the resistance of BGH-2127 late blight.