TITLE:
In Vitro Priming of Sugarcane Varieties (RB966928 and RB867515)
AUTHORS:
Rafaela Pereira Duarte, Rodrigo Kelson Silva Rezende, Fernanda Pinto, Maílson Vieira Jesus, Izaias Rodrigues da Silva Junior, Ana Maria Scoton
KEYWORDS:
Salinity Stress, Micropropagation, Saccharum officinarum L.
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.4,
March
20,
2018
ABSTRACT: Sugarcane,
as a glycophyte, shows sensitivity to saline soils at various stages of its
growth. The aim of this study was to evaluate the in vitro priming response in two sugarcane varieties (RB966928 and
RB867515). Micropropagated plants, from meristems, received priming treatments
by adding the salts (NaCl and KCl) in different concentrations (0.0; 12.5; 25.0
and 50.0 mM), in the MS medium. Subsequently, the plants were cultivated in
rooting medium without addition of salts, acclimatized and submitted to
gradient ex vitro saline stress with
20 → 40 and
60 mM, of each salt, for 30 days. The analyzed variables were dry matter of
shoot and root, number of tillers and estimation of chlorophyll content. The
experiment was carried out in a 2 × 2 × 4 factorial arrangement, in a completely
randomized experimental design. Twenty replicates were used throughout the
experiment. Data were submitted to analysis of variance and regression and the
means were compared by Tukey’s test,
at a 5% probability level. The priming treatments presented a significant
effect, with triple interaction, in the chlorophyll index. In the treatment
with NaCl, the variety RB966928 showed an increase in the chlorophyll index
with the increase of treatment levels, up to an optimal limit of 31.47 mM. On
the other hand, the variety RB867515 showed decreasing in chlorophyll index. In
contrast, in KCl treatment, the variety RB867515 presented the increase at the
chlorophyll index with the maximum point of 25 mM. For the variables, shoot dry
matter (SDM) and root dry matter (RDM) there was a significant difference (p 0.05 and p 0.01, respectively) only
between the varieties. The variety RB966928 presented higher SDM and RDM in
relation to the variety RB867515. Studies are recommended with increasing the
duration of the priming treatments and more detailed study of the culture
throughout its productive cycle.