TITLE:
Explore the Relationship between Insulin Resistance and PGC1α in PCOS Mice
AUTHORS:
Lei Chen, Xin Tao, Shuqi Ge, Lisi Cai, Xuanying Deng
KEYWORDS:
PCOS, Mice, Insulin Resistance, PGC1α
JOURNAL NAME:
Open Journal of Endocrine and Metabolic Diseases,
Vol.8 No.3,
March
16,
2018
ABSTRACT: Objective: To investigate the relationship between AMPKα, PGCα, insulin
resistance and reproductive function in PCOS mice and to find out the molecular
mechanisms and potential therapeutic molecular targets of pathogenesis of PCOS. Methods: The PCOS mouse model was established by DHEA administering with
Balb/c mice. And after AMPK agonist AICAR and inhibitor Compound C
intervention, Fasting blood glucose, fasting insulin and testosterone levels
were observed. The HOMA index was calculated. The changes of PGCα expression in ovarian tissue were
observed by western blot and immunohistochemistry to determine the relationship
between insulin resistance and PGCα in PCOS mice. Results: Western blot and immunohistochemistry showed that
PGC1α protein was expressed in the
ovary of mice and the expression of PGC1α was negatively correlated with AMPKα in our study. Compared with the control group, the expression of PGC1α in the ovaries of the mice in PCOS
group was significantly increased (P α in PCOS + AICAR group was lower than
that in PCOS group (P α in PCOS mice exposed to AMPK inhibitor Compound C also decreased
compared with PCOS group (P Conclusion: In ovarian tissue, the insulin resistance-related AMPKα pathway in PCOS mice may be negatively correlated with PGCα.