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Rodríguez, N., Ortiz, C., Herrero, A. and Sarabia, L. (2007) Performance Characteristics According to Commission Decision 2002/657/EC in the Fluorimetric Determination of Tetracycline in the Absence and in the Presence of Magnesium. Luminescence, 22, 518-526.
https://doi.org/10.1002/bio.993

has been cited by the following article:

• TITLE: Development and Validation of a Spectrofluorimetric Method for the Assay of Tetracycline in Capsules

  AUTHORS: Lucien Murhula Namegabe, Serigne Omar Sarr, Yérim Mbagnick Diop

  KEYWORDS: Validation, Spectrofluorimetry, Tetracycline

  JOURNAL NAME: American Journal of Analytical Chemistry, Vol.9 No.3, March 15, 2018

  ABSTRACT: The purpose of this study is to develop and validate a method for the analysis of tetracycline capsules by spectrofluorimetry. A pH 9 borate buffer was used as diluent of tetracycline after reaction with magnesium salt at the excitation wavelength of 372 nm and 516 nm of emission. A linear response was observed between 0.25 μg/mL and 1.5 μg/mL with a correlation coefficient (R) of 0.9998. The detection and quantification limits found are 0.0125 μg/mL and 0.0412 μg/mL respectively. The proposed method proved trueness with a recovery between 99.88% and 101.10%. The relative standard deviations of repeatability and intermediate precision found ≤2.88% reflected a good precision of the method. The proposed method is therefore valid within the limits of 90% to 110%. The proposed method was applied to the quality control of 9 tetracycline samples from market and gave results in accordance with the pharmacopoeia standards.