TITLE:
Comparison of Chondrogenic Ability between Mesenchymal Stem Cells and Buffy Coat in Vitro
AUTHORS:
Longhao Jin, Zhongye Zhang, Yanqun Liu, Kumar Gurung, Yongjun Jin, Yuanming He, Yu Jin, Zhehu Jin
KEYWORDS:
Chondrogenic, Buffy Coat, Mesenchymal Stem Cells
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.6 No.3,
March
14,
2018
ABSTRACT: Objective: In vitro comparison of chondrogenic
differentiation ability of bone marrow
extracted mesenchymal stem cells (MSCs) and buffy coat. Methods: MSCs of New Zealand white rabbits were cultured in vitro and adherent cells were passaged. The cells were
inoculated on polyglycolic acid (PGA) scaffold (3 mm in diameter and 2 mm in
height) liquid for 21 days. Under the same conditions, without subculture, buffy coat was directly inoculated into the
cell scaffold. The degree of chondrogenic differentiation was compared by
Safranin-Ostaining, histological
scoring and biochemical functional detection. Results: The
chondrogenic differentiation ability of the buffy coat group was better than
that of the MSC group. Safranin-Ostain was stronger in buffy coat group than
in MSC group. The Bern Score was also higher in the buffy coat group than in
the MSC group and the total amount of sulfated glycosaminoglycans (GAGs) in
buffy coat group was higher than that in MSC group. Conclusion: The
chondrogenic differentiation ability of buffy coat is higher than that of MSC.
Through this result, it was found buffy coat can also differentiate into
sub-cartilage without stimulating growth factor. Thus, buffy coat can make a
great source in artificial cartilage engineering.