TITLE:
SCFA Profile of Rice RS Fermentation by Colonic Microbiota, Clostridium butyricum BCC B2571, and Eubacterium rectale DSM 17629
AUTHORS:
Donald John Calvien Hutabarat, Fransisca Rungkat Zakaria, Endang Yuli Purwani, Maggy Thenawidjaja Suhartono
KEYWORDS:
Resistant Starch, Colonic Microbiota, Clostridium butyricum BCC-B2571, Eubacterium rectale DSM 17629, SCFA
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.9 No.2,
February
28,
2018
ABSTRACT: Resistant
starch type 3 (RS3) produced from high amylose food sources through
retrogradation or enzymatic process is known to have physiological function as
dietary fiber. Fermentation of RS3 by colonic microorganisms produced SCFA
(acetate, propionate, and butyrate), maintained the
health of colon, balance of gut microbiota, preventing inflammatory bowel
diseases (IBD) and colon cancer. RS3 in
this study was produced from IR-42 and Inpari-16
broken rice by enzymatic treatment (combination of amylase-pullulanase).
The Resistant Starch was fermented for 12 and 24 h by colonic microbiota
(extracted from healthy human subject), Clostiridium
butyricum BCC-B2571, or Eubacterium
rectale DSM
17629. SCFA produced was analyzed by gas chromatography. Treatment by
amylase-pullulanase combination was advantageous to increase their RS3 content.
The result showed that after enzymatic process, the RS3 content of IR-42
(41.13%) was not significantly different (p 0.05) from that of Inpari-16 (37.70%). High
concentration of acetate (82.5 mM) and propionate (7.5 mM) were produced by colonic microbiota after 12 h
fermentation and best concentration of butyrate (6.8 mM) was produced by
colonic microbiota after 24 h fermentation. It is clear that utilization of
colonic microbiota rather than single strain was better in the production of
SCFA.