TITLE:
A Method to Improve the Embryogenesis Rate of Banana Somatic Cell Embryogenesis
AUTHORS:
Shenghe Chang, Zhengjing Wu, Wei Sun, Fei Lin, Qi Zeng, Jingyi Zhang, Lan Qiao, Haiyan Shu
KEYWORDS:
Protein Transduction, Banana, Embryogenic Callus, WIND1
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.9 No.3,
February
27,
2018
ABSTRACT: Getting embryogenic callus is the first step for
banana transformation. Enhancing embryonic-callus-rate is important for banana
molecular breeding. In this paper, WIND1, an AP2/ERF transcription factor was
added into the embryonic-callus-inducing medium with poly-Arg and nuclear
localization signal (NLS). The embryogenic-callus-induced rate of the immature
male florescence cultured on the media containing the recombinant protein
Arg9-NLS-WIND1 was significantly higher than that of the immature male
florescence cultured on the control media. Medium containing 0.01% of the
protein Arg9-NLS-WIND1 had the best effect for inducing the embryogenesis of
the immature male inflorescence. On the medium containing 0.01% of the protein
Arg9-NLS-WIND1, 11.5% of the immature male flowers can develop embryonic callus.
On the medium containing Arg9-NLS-WIND1, the protein might be transferred into
cytoplasm of the explants by endocytosis due to the interaction between the
poly-Arg transduction domain and the plasma membrane. After it entered the
nucleus guided by NLS and bind with the target DNA domain, the somatic cell
embryogenesis reactions were initiated and the embryonic callus formed.