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Ferradini, N., Giancaspro, A., Nicolia, A., Gadaleta, A., Veronesi, F. and Rosellini, D. (2016) Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes. In: MacDonald, J., Kolotilin, I. and Menassa, R., Eds., Protocol Recombinant Proteins from Plants: Methods and Protocols, Springer, Berlin, 89-98.

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• TITLE: Recent Progress of Transgenic Technology Development for Alfalfa

  AUTHORS: Wenting Liu, Banyar Aung, Abdelali Hannoufa, Tim Xing, Lining Tian

  KEYWORDS: Medicago sativa, Transformation, Tissue Culture

  JOURNAL NAME: American Journal of Plant Sciences, Vol.9 No.3, February 26, 2018

  ABSTRACT: Alfalfa (Medicago sativa L.) is an important leguminous crop worldwide and it has important roles in different aspects of the agriculture system, including livestock feed, crop soil conservation, and improvement of soil nitrogen supply. As a perennial crop, alfalfa breeding for trait improvement takes longer periods of time compared to many other crops, therefore, genetic engineering is a faster route for alfalfa trait modification and improvement. Alfalfa was a first crop in which somatic embryogenesis was developed. Alfalfa was also a pioneering crop in which transgenic technology was developed and applied for trait improvement. Transgenic technology in alfalfa has since advanced steadily in various areas. In this review, we update the recent research progress in alfalfa genetic engineering, focusing on genetic transformation and use of transgenic technology for trait improvement and new trait development.