TITLE:
miR-125b, miR-200c Are Correlated with the Severity of Interstitial Lung Disease in Dermatomyositis/Polymyositis
AUTHORS:
Zhen Jiang, Jinhui Tao, Xiangpei Li
KEYWORDS:
Dermatomyositis/Polymyositis, Interstitial Lung Disease, miR-125b, miR-200c, Severity
JOURNAL NAME:
Open Journal of Rheumatology and Autoimmune Diseases,
Vol.8 No.1,
February
1,
2018
ABSTRACT: Objective: To explore the correlations between miR-125b, miR-200c, and the
severity of interstitial lung disease associated with dermatomyositis/polymyositis
(DM/PM-ILD). Methods: 30 consecutive patients with DM/PM and 23 healthy
controls were recruited into current study. Anti-JO-1, anti-SSA, muscle
enzymes, the data of chest HRCT and pulmonary function test were collected. 9
consecutive DM/PM-ILD patients underwent bronchoalveolar lavage (BAL). TGF-β1 and surfactant protein D (SP-D) in BAL
fluid (BALF) and plasma were detected by ELISA. miR-125b and miR-200c in PBMCs
and bronchoalveolar cells were detected by QRT-PCR. All patients were
classified into three groups: Mild or non-ILD group, moderate ILD group, and
severe ILD group. The correlations between miRNAs and the severity of ILD, the
lung damage markers, auto-antibodies, were analyzed. Results: The levels of miR-125b and miR-200c in bronchoalveolar cells
were higher than in PBMCs, and the levels of TGF-β1 and SP-D were higher in BALF than in plasma in DM/PM-ILD
patients. There were positive correlations between miR-125b, miR-200c in
bronchoalveolar cells and SP-D in BALF. The levels of miR-125b and miR-200c in severe ILD
group were higher than in mild or non-ILD and moderate ILD groups. There were
negative correlations between miR-125b, miR-200c, and FEV1, and between
miR-200c and DLCO. The patients with anti-JO-1 antibody had higher levels of
miR-125b and miR-200c, and had more severe
condition of ILD. Conclusion: miR-125b and miR-200c were positively correlated
with the lung damage and severity of ILD in DM/PM, which could be important
markers for judgement of disease condition in clinic.