TITLE:
cDNA Cloning of Paramyosin from Several Kinds of Squid Mantle Muscle
AUTHORS:
Takayuki Kajita, Yoshiko Takeda, Saki Yoshida, Koki Yamada, Masahiro Matsumiya, Hideto Fukushima
KEYWORDS:
Paramyosin, Squid, cDNA Cloning, Mantle Muscle, Phylogenetic Analysis
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.9 No.1,
January
29,
2018
ABSTRACT: Paramyosin
is a rod-shaped muscle protein found exclusively in invertebrates, with α-helices
coiled around each other to form a coiled-coil structure. Marine organisms in
which the primary structure of paramyosin has been determined are mollusks,
including abalone (Haliotis discus),
mussels (Mytilus galloprovincialis),
octopus (Octopus bimaculoides), and
oyster (Crassostrea gigas). In
contrast, the primary structure of squid paramyosin, which is of particular interest,
has yet to be reported. In the present study, cDNA cloning of paramyosins from
four squid species, the neon flying squid (Ommastrephes bartramii), the
Humboldt squid (Dosidicus gigas), the
golden cuttlefish (Sepia esculenta), and
the clawed armhook squid (Gonatus onyx), was
performed to determine the following: the 2605-bp O. bartramii paramyosin gene containing a 2574-bp open reading frame (ORF), the 2691-bp D. gigas paramyosin gene containing a 2640-bp ORF, the 2631-bp S. esculenta paramyosin gene containing a 2574 ORF, and the 2609-bp G. onyx paramyosin gene containing a 2574-bp ORF. The primary structure of the four
squid paramyosins was found to contain heptad repeats and an ACD (assembly competence domain), which are characteristic
of a coiled coil. A phylogenetic analysis was performed with paramyosin
sequences from species including the four squid species examined in this study,
the results of which indicated that the four squid paramyosins form a group independent
from the paramyosins of other species, to which octopus paramyosins are
closest.