TITLE:
Myricetin and Hesperidin Inhibit Cerebral Thrombogenesis and Atherogenesis in Apoe-/- and Ldlr-/- Mice
AUTHORS:
Yasuto Sasaki, Kanae Hyodo, Ayana Hoshino, Eri Kisa, Koichi Matsuda, Yoko Horikawa, John C. Giddings
KEYWORDS:
Myricetin, Hesperidin, Atherosclerosis, Apoe-/- and Ldlr-/- Mice, Thrombosis
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.9 No.1,
January
22,
2018
ABSTRACT: Flavonoids have been reported to possess strong antioxidant activities that
moderate endothelial dysfunction and demonstrate protective effects on cardiovascular
disease. Our previous studies confirmed that flavonoids, including
hesperidin, naringin and nobiletin, inhibited thrombogenesis and hypertension
in stroke prone spontaneously hypertensive rats (SHRSP) by protecting
the endothelium from the adverse effects of free radical formation. We have
now further investigated the protective effects of myricetin and hesperidin on
cerebral thrombosis and atherogenesis in apolipoprotein E (apoE) and lowdensity
lipoprotein receptor (LDLR) deficient (Apoe-/- and Ldlr-/- double
knockout) mice. Three groups of mice were fed high fat diet alone and high
fat diet mixed with myricetin (100 mg/kg/day and 200 mg/kg/day) or glucosyl
hesperidin (G-hesperidin; 250 mg/kg/day and 500 mg/kg/day) for 8 weeks.
There were no differences in body weight related to administration of the flavonoids.
Thrombotic tendency was assessed using a He-Ne laser technique in
the murine cerebral pial vessels. In addition, atherogenesis was quantified
histologically after dissection of the aorta from each mouse and staining with
Oil Red O solution. The percentages of stained area to whole area of dissected
aorta were calculated as indices of anti-atherogenic activity. Both myricetin
and G-hesperidin significantly inhibited thrombogenesis in vivo and significantly
inhibited atherogenesis compared to control mice (p