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Yu, J., Wang, A., Tang, Z., Henry, J., Li-Ping Lee, B., Zhu, Y., Yuan, F., Huang, F. and Li, S. (2012) The Effect of Stromal Cell-Derived Factor-1alpha/Heparin Coating of Biodegradable Vascular Grafts on the Recruitment of Both Endothelial and Smooth Muscle Progenitor Cells for Accelerated Regeneration. Biomaterials, 33, 8062-8074.
https://doi.org/10.1016/j.biomaterials.2012.07.042

has been cited by the following article:

• TITLE: Chemotactic Activity of Site-Specific Multivalent Conjugates of Stromal Cell-Derived Factor 1α on Branched Nanoparticles

  AUTHORS: Yu-Fang Hsieh, Fang Huang, Shyam Patel, Song Li

  KEYWORDS: SDF1α Delivery, Cell Recruitment, Site-Specific Protein Ligation, Multivalent Protein Conjugates

  JOURNAL NAME: Journal of Biomaterials and Nanobiotechnology, Vol.9 No.1, January 11, 2018

  ABSTRACT: Stromal cell-derived factor 1α (SDF1α) is a potent chemokine for the recruitment of stem cells. A challenge is to maintain its activity and control its release. In this study, we engineered a recombinant cysteine-SDF1α (cysSDF1α) protein, and performed multivalent conjugation of cysSDF1 through the maleimide functional group to two forms of branched nanoparticles: multi-arm poly (ethylene glycol) (MA-PEG) and hyaluronic acid (HA). We characterized the chemotactic activity of the conjugates, and determined how the molecular weight (MW) of MA-PEG and HA affected the chemotactic activity. CysSDF1α had similar efficiency to wild-type SDF1α in cell recruitment. Multivalent conjugation of cysSDF1α to low MW MA-PEG (~18 nm) did not significantly affect the chemotactic activity, while the conjugation of cysSDF1α to high MW MA-PEG (~72 nm) lowered the efficiency, possibly due to the larger spacing between conjugated SDF1α molecules. HA has a linear backbone and a high density of multivalent binding sites; however, the chemotactic activity of HA-linked cys-SDF1α was much lower, which further decreased with the increase of HA MW from 200 kDa (~0.78 μm) to 700 kDa (~2.7 μm). Digestion of HA into smaller fragments using hyaluronidase partially recovered the chemotactic activity of cysSDF1α, suggesting that high MW HA might exert steric hindrance for SDF1α binding to its receptors on cell surface and that HA could be used as a depot for SDF1α storage and release. These results demonstrate that multivalent conjugates of SDF1α to nanoparticles may be used to engineer SDF1α delivery for cell recruitment and tissue regeneration.