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Chen, A. and Zhang, Z.J. (1994) Research on Avian Influenza: Chicken I A Avian Influenza Virus Isolation and Identification of Serological. Chinese Veterinary Journal, 22, 3-5.

has been cited by the following article:

• TITLE: Establishment and Application of Digital RT-PCR Assay for Detection of Avian Influenza Virus H9 Subtype

  AUTHORS: Bin Wu, Lin Zhang, Liming Su, Huijun Zhao, Xiaoping Cai

  KEYWORDS: Avian Influenza Virus H9 Subtype (H9), Digital RT-PCR, Real-Time Quantitative RT-PCR, Sensitivity, Specificity

  JOURNAL NAME: Advances in Microbiology, Vol.7 No.11, November 28, 2017

  ABSTRACT: A digital RT-PCR method for rapid detection of H9 subtype influenza was established by comparing the two methods of digital RT-PCR and real-time quantitative RT-PCR. The sensitivity, specificity and reproducibility of the two methods for H9 were determined by gradient dilution using the same pair of primers and probes. Both methods were able to detect 104 times diluted H9 pathogens, while digital RT-PCR could detect H9 in single droplets, and its sensitivity was higher than real-time quantitative RT-PCR. At the same time, the specificities of both methods were very strong, with no amplification reactions for H3N2, H4N2, H6N2. The reproducibility of the two methods were also good. Digital RT-PCR has a higher sensitivity than real-time quantitative RT-PCR and could play an important role in the rapid detection of H9 subtype influenza virus.