TITLE:
Concentrations of Silver Nitrate in the In Vitro Development and Conservation of Passiflora gibertii N. E. Brown
AUTHORS:
Gláucia Amorim Faria, Lucas Menezes Felizardo, Antonio Flávio Arruda Ferreira, Paula Suares Rocha, Aline Namie Suzuki, Antônio da Silva Souza, Tatiana Góes Junghans, Maria A. P. C. Costa, Ana Patricia Bastos Peixoto, Augusto Ramalho de Morais, Beatriz Garcia Lopes, Tiago Almeida de Oliveira
KEYWORDS:
Passion Fruits, Tropical Fruits, Plant Propagation, Reduced Growth, Ethylene
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.12,
November
13,
2017
ABSTRACT: The aim of this study was to evaluate the effects of
silver nitrate in the in vitro development and conservation of passion fruit plants. Experiments were carried
out at the Laboratory of Culture and Tissues, in the National Cassava and
Fruits Research Center (EMBRAPA), using microcuttings of Passiflora gibertii N. E. Br., previously cultivated in vitro.
The microcuttings were placed in MS media supplemented with silver nitrate at
concentrations of 0, 1, 2, 4 and 8 mg·L-1, and, during 30, 60 and 90
days, the following variables were identified: Shooting length (cm), number of
roots, number and coloration of leaves. A completely randomized design with 30 replications
in a split-plot scheme was employed. Polynomial regression equations were
adjusted in analysis of variance to compare averages of treatments. The
obtained results support the assumption of high sensitivity of passion fruit
tissues to ethylene, which reflects in the lost of vigor and in the induction
of culture senescence by its accumulation. Based on obtained results, and in
order to mitigate this problem, it is possible to indicate an addition of 2 mg·L-1of silver nitrate in culture media, for the micropropagation of passion fruit
plant, allowing the controlling of the atmosphere inside the culture test
tubes, during the establishment of explants and other steps of the process.