TITLE:
Antimicrobial Activity of the Essential Oil from Hyptis carpinifolia Benth
AUTHORS:
Karen Caroline Camargo, Luís Roberto Batista, Paulo Estevão de Souza, Maria Luisa Teixeira, Thaís Aparecida Sales, Vanuzia Rodrigues Fernandes Ferreira, Jéssica Oliveira e Nogueira, Maísa Lamounier Magalhães, Alex Rodrigues Silva Caetano, David Lee Nelson, Maria das Graças Cardoso
KEYWORDS:
Essential Oils, Antimicrobial Activity
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.11,
October
27,
2017
ABSTRACT: Essential oils are secondary metabolites whose
biological properties have been studied with emphasis on antimicrobial
activity. Hyptis carpinifolia Benth
(Rosmaninho) is used in folk medicine in the fight against colds and
rheumatism. The objective of this study was to evaluate the antimicrobial
activity of the essential oil from H.
carpinifolia. The essential oil was extracted by hydrodistillation using a
modified Clevenger apparatus. The biological activity was determined using the
Agar Cavity Diffusion technique to evaluate the effect of concentrations of
500, 250, 125, 62.5, 31.25, 15.62, 7.81 and 3.9 μg·mL-1 on the bacteria Escherichia coli, Listeria monocytogenes, Salmonella Choleraesuis, Staphylococcus aureus and Pseudomonas aeruginosa. The fungicidal
potential was evaluated by the method of dilution in agar, and the percentage
inhibition of mycelial growth of Alternaria
alternata, Botrytis cinerea and Fusarium oxysporum was evaluated. The
dilutions tested were 1000, 750, 500, 250 and 100 μL·L-1.
The minimum inhibitory concentrations (MIC) of the essential oil for L. monocytogenes, S. aureus and E. coli were 31.25; 15.62 and 62.5 μg·mL-1, respectively. The MIC
was 500 μg·mL-1 for Salmonella Choleraesuis and P. aeruginosa. The percentage inhibitions of mycelial growth of B. cinerea and A. alternate were 67% and 33%, respectively, at the dilution of 750
μL·L-1. The essential oil inhibited about 48% of the
mycelial growth of F. oxysporum, at
the dilution of 1000 μL·L-1. The essential oil of H. carpinifolia presented biological
activity against all the microorganisms evaluated.