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Saz, J.V., Beltrán, M., Díaz, A., Agulla, A., Merino, F.J., Villasante, P.A. and Velasco, A.C. (1987) Enzyme-Linked Immunosorbent Assay for Diagnosis of Brucellosis. Clinical Microbiology, 6, 71-74. https://doi.org/10.1007/BF02097200

has been cited by the following article:

• TITLE: Titers of Anti-Brucella Antibodies by Enzyme Linked Immunosorbant Assay in Vaccinated and Unvaccinated Brucellosis Infected Cattle

  AUTHORS: Sugandha Raj, Hari Mohan Saxena, Sikh Tejinder Singh

  KEYWORDS: Antibody Titer, Brucella, Brucellosis, Bovine, ELISA

  JOURNAL NAME: Open Journal of Veterinary Medicine, Vol.7 No.10, October 25, 2017

  ABSTRACT: Brucellosis is an important re-emerging zoonotic disease caused by Brucella organisms. In the absence of a Differentiation of Infected from Vaccinated Animal (DIVA) assay for bovine Brucellosis, it becomes difficult to assess whether the anti-Brucella antibody response in an animal is due to vaccination or infection. We compared the anti-Brucella antibody titers of naturally Brucellosis affected unvaccinated cows, previously vaccinated infected cows, normal healthy vaccinated cows and healthy unvaccinated calves. The titers of anti-Brucella antibodies were estimated by indirect ELISA. The mean titer (log10) was found to be 1.518 ± 0.005 in case of naturally Brucellosis affected cattle which had been vaccinated during calf hood. The mean titer in case of naturally infected cattle which had never been vaccinated was 1.5441 ± 0.005. The mean titer in healthy unaffected cattle vaccinated during calf hood was 1.504 ± 0.002 and that of unvaccinated healthy calves was 0.560 ± 0.016. It was interesting to find that the antibody titers in naturally affected cattle which had never been vaccinated were very significantly (p