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Silva, N.R.A., Luna, M.A.C., Santiago, A.L.C.M.A., Franco, L.O., Silva, G.K.B., Souza, P.M., Okada, K., Albuquerque, C.D.C., Silva, C.A.A. and Takaki, G.M.C. (2014) Biosurfactant and Bioemulsifier Produced by a Promising Cunninghamella echinulata Isolated from Caatinga Soil in the Northeast of Brazil. International Journal of Molecular Sciences, 15, 15377-15395.
https://doi.org/10.3390/ijms150915377

has been cited by the following article:

• TITLE: Optimization Techniques and Development of Neural Models Applied in Biosurfactant Production by Bacillus subtilis Using Alternative Substrates

  AUTHORS: Juliana Ferrari Ferreira Secato, Brunno Ferreira dos Santos, Alexandre Nunes Ponezi, Elias Basile Tambourgi

  KEYWORDS: Biosurfactant, Bacillus subtilis, Response Surface Methodology, Artificial Neural Network, Oil Spreading, Waste Management

  JOURNAL NAME: Advances in Bioscience and Biotechnology, Vol.8 No.10, October 13, 2017

  ABSTRACT: Bacillus subtilis was investigated as production of biosurfactant using a combination based on waste of candy industry and glycerol from biodiesel production process as only substrate. The experimental design chosen for optimization by response surface methodology was a central composite rotatable design (CCRD) and dry weight (DW) and crude biosurfactant (CB) concentrations were selected as responses in analysis. Two techniques were implemented response surface methodology (RSM) and artificial neural network (ANN). First challenge of study was to assess the effects of the interactions between variables and reach optimum values. With the CCRD results, RSM and ANN models were developed, optimizing the production of biosurfactant. The correlation coefficients (R2) of RSM models explained 88% for DW and 73% for CB of the interactions among substrate concentrations, while ANN models explained 99% for DW and 98% for CB, demonstrating that developed ANN models were more accurate and consistent in predicting optimized conditions than RSM model. The maximum DW and CB produced in the optimum conditions were 25.60 ± 5.0 g/L and 668 ± 40 mg/L, respectively. The crude biosurfactant also showed applications in cases of oil spreading in water due to clear zone produced in Petri dishes assays.