TITLE:
Detection of Antibody in Dogs with Blastomycosis Using Blastomyces dermatitidis Yeast Phase Lysate Antigens
AUTHORS:
Bryn C. Kennell, Gene M. Scalarone, Lilliana Hoyos-Carvajal, Moytri RoyChowdhury
KEYWORDS:
Blastomyces Dermatitidis, Yeast Phase Lysate Antigen, Blastomycosis
JOURNAL NAME:
Open Journal of Veterinary Medicine,
Vol.7 No.9,
September
29,
2017
ABSTRACT: The objective of our study was to compare two B. dermatitidis yeast phase lysate antigens [ERC-2, dog Wisconsin; 85, soil Georgia, ATCC 56,920] for detecting antibody in 38 serum specimens [pre-treatment, 30-day, and 60-day post treatment] from dogs with diagnosed blastomycosis. The mean absorbance values obtained with the two antigens (N = 38) were ERC-2 = 2.359 and 85 = 2.189. The mean absorbance values when the sera were divided into the three treatment groups were as follows pre-treatment: Isolate ERC-2 had an absorbance value of 2.418; Isolate 85 had an absorbance value of 2.688, 30-day post treatment: ERC-2 had an absorbance value of 2.452; 85 had an absorbance value of 2.303 and 60-day post treatment: ERC-2 had an absorbance value of 2.150; 85 had an absorbance value of 2.073 with the mean absorbance values of all treatment groups were ERC-2: 2.229 and 85: 2.141. This study indicates the potential for further evaluations of the two lysate antigens with regard to antibody detection in dog sera with the ERC-2 reagent slightly more reactive than the 85 lysate antigen.