TITLE:
Reduced Sensitivity of Campomanesia adamantium (Cambess.) O. Berg Seeds to Desiccation: Effects of Polyethylene Glycol and Abscisic Acid
AUTHORS:
Daiane Mugnol Dresch, Tathiana Elisa Masetto, Tatiane Sanches Jeromini, Silvana De Paula Quintão Scalon
KEYWORDS:
Abscisic Acid, Osmotic Conditioning, Polyethylene Glycol, Water Stress
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.10,
September
22,
2017
ABSTRACT: The Campomanesia adamantium is a threatened species from Brazil Savannah which seeds are desiccation-sensitive and do not withstand storage. This study aimed to reduce the sensitivity of Campomanesia adamantium seeds to desiccation using polyethylene glycol (PEG) and abscisic acid (ABA).
Initially, seeds were subjected to PEG (0, -1.48, and -2.04 MPa) with or
without ABA (100 μM) during 120 h, followed fast drying (silica gel) or slow
drying (laboratory environment), at 20%, 15%, and 10% moisture content. In the
second experiment, the seeds were PEG treated (-1.48 MPa) which provided the
best results in the first experiment; the seeds were then subjected to
different incubation times in PEG (30, 60, 90, or 120 h) and ABA (0, 10ˉ3,
10ˉ4, and 10ˉ5 μM), following
the seeds were fast dried at 15% moisture content. The slow drying should be
avoided, even in seeds previously subjected to osmotic conditioning with or
without ABA. Seeds submitted to PEG treatment (-1.48 MPa/120h) without ABA and
PEG (-1.48 MPa) with 10ˉ3 or 10ˉ4 μM of ABA (90 h),
followed by fast drying at 15% moisture content showed reduction of desiccation sensitivity and high germination and vigor
when compared to the other treatments.