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Knapp, E., Hanzer, V., Weiss H, H., da Camara Machado, A., Weiss, B., Wang, Q., Katinger, H. and Laimer da Camara Machado, M. (1995) New Aspects of Virus Elimination in Fruit Trees. Acta Horticulturae, 386, 409-418.
https://doi.org/10.17660/ActaHortic.1995.386.56

has been cited by the following article:

• TITLE: Fast and Effective Thermotherapy Treatment for In Vitro Virus Eradication in Apple and Pear Trees

  AUTHORS: Analí Lizárraga, Javier Ascasíbar, María Luz González

  KEYWORDS: Heat Therapy, Meristem Culture, ACLSV, ApMV, Malus, Pyrus

  JOURNAL NAME: American Journal of Plant Sciences, Vol.8 No.10, September 20, 2017

  ABSTRACT: Heat therapy followed by the isolation and in vitro culture of apical meristems is a suitable procedure for virus eradication. However, the period of heat treatment is usually long (28 - 50 days) and the yield of viable plants free of viruses after treatment is often low (in vitro shoots using a temperature gradient from 25℃ to 40℃ increasing 1℃ per day for a shorter period of time (18 days) with the posterior isolation and culture of apical meristems. All DNA samples analyzed, obtained from plants developed from meristems, were 100% free of ApMV and almost 90% free of ACLSV. With this in vitro procedure combined we obtained a good yield of tested plants free of viruses. Our method is fast and effective and it could be also useful to eradicate these and other viruses in other fruit trees.