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Mink, G.I., Wample, R. and Howell, W.E. (1998) Heat Treatment of Perennial Plants to Eliminate Phytoplasmas, Viruses, and Viroids While Maintaining Plant Survival. In: Hadidi, A., Ed., Plant Virus Disease Control, Springer, St. Paul.

has been cited by the following article:

• TITLE: Fast and Effective Thermotherapy Treatment for In Vitro Virus Eradication in Apple and Pear Trees

  AUTHORS: Analí Lizárraga, Javier Ascasíbar, María Luz González

  KEYWORDS: Heat Therapy, Meristem Culture, ACLSV, ApMV, Malus, Pyrus

  JOURNAL NAME: American Journal of Plant Sciences, Vol.8 No.10, September 20, 2017

  ABSTRACT: Heat therapy followed by the isolation and in vitro culture of apical meristems is a suitable procedure for virus eradication. However, the period of heat treatment is usually long (28 - 50 days) and the yield of viable plants free of viruses after treatment is often low (in vitro shoots using a temperature gradient from 25℃ to 40℃ increasing 1℃ per day for a shorter period of time (18 days) with the posterior isolation and culture of apical meristems. All DNA samples analyzed, obtained from plants developed from meristems, were 100% free of ApMV and almost 90% free of ACLSV. With this in vitro procedure combined we obtained a good yield of tested plants free of viruses. Our method is fast and effective and it could be also useful to eradicate these and other viruses in other fruit trees.