TITLE:
Biological Control of Pyricularia oryzae Using Antifungal Compounds Produced by Aspergillus niger
AUTHORS:
Ali Abdulameer Idan, Kamaruzaman Sijam, Jugah Kadir, Tavga Sulaiman Rashid, Hayman Kakakhan Awla, Wael Alsultan
KEYWORDS:
Blast Disease, Aspergillus sp., Pyricularia oryzae, Antifungal Compounds, GC-MS
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.10,
September
15,
2017
ABSTRACT: Aspergillus
spp. has been widely found as useful microorganism in
biotechnology. They have a high ability in the production of secondary
metabolites. Therefore, isolates of Aspergillus were isolated from healthy rice field located in Selangor State/Malaysia. The obtained strain
(UPMZ01) was conducted against Pyricularia oryzea by applying dual culture and culture filtrate technique. The antagonism
of strain UPMZ01 in the dual culture was 81.326% inhibition percentage against P.
oryzae given the optimum inhibitory percentage 100% at all concentration of secondary metabolites aged 14 days. The isolate (UPMZ01) was identified as Aspergillus niger with accession number
(KY698415). The environment factors such as pH and temperature
influencing on production of secondary
metabolites. The results were shown that pH at level 5.0 and temperature
between 21℃to 29℃is the optimum condition for A. niger to produce efficient antifungal metabolites which
given 100% PIGR against blast pathogen. The secondary metabolites compounds
were identified by Gas Chromatography-Mass
Spectrometry (GC-MS). Fifteen compounds were recognized as major compounds which may have the possibility of possessing antifungal
characteristics. Most of
identified compounds are Oleic Acid, n-Hexadecanoic acid, Hexose, Glycerol,
Stearic acid, Tetradecanoic acid, Dodecanoic acid and 5-Hydrxoymethylfurfural.