TITLE:
Effects of Pyridoxine on Selected Appetite Regulating Peptides mRNA Expression in Hypothalamic PVN/ARC Nuclei and Gastrointestinal Tract Tissues
AUTHORS:
Lei Liu, Haoqi Wang, Haitao Sun, Chunyan Fu, Hongli Liu, Yuqing Sun, Xianghua Xu, Weiji Chen, Wenqiang Wu, Fuchang Li
KEYWORDS:
Pyridoxine, Brain-Gut Peptides, Appetite Control
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.8 No.9,
September
13,
2017
ABSTRACT: An experiment was
conducted to investigate the effect of dietary pyridoxine on the gene
expression of appetite-regulating peptides in the hypothalamus and
gastrointestinal tract of rabbits. Thirty-two rabbits were randomly divided
into 2 treatments for 8 weeks (16 replicates/group and 1 rabbit/replicate). The
treatments were fed a basal diet (control, measured pyridoxine content is 4.51
mg/kg) and the basal diet with a pyridoxine supplementation at 10 mg/kg (pyridoxine,
measured pyridoxine content is 14.64 mg/kg). The results showed that dietary pyridoxine
did not significantly alter the mRNA levels of neuropeptide Y, agouti related
peptide, pro-opiomelanocortin and cocaine, amphetamine regulated transcript, peptide
YY and cholecystokinin in arcuate nucleus, peptide YY in jejunum and ileum, and
cholecystokinin in duodenum, jejunum and ileum (P > 0.05). Compared
with the control, the mRNA levels of corticotropin-releasing hormone and
melanocortin 4 receptor in paraventricular nuclei and peptide YY in duodenum were
significantly decreased after pyridoxine treatment (P 0.05). In conclusion,
the appetite genes of melanocortin 4 receptor and corticotropin-releasing
hormone in paraventricular nuclei and peptide YY in duodenum are involved in the
pyridoxine-caused hyperphagia.