TITLE:
Derivative Spectrophotometric and Isocratic High Performance Liquid Chromatographic Methods for Simultaneous Determination of Repaglinide and Metformin Hydrochloride in Pharmaceutical Preparations
AUTHORS:
Serap Sağlık Aslan, Berna Yılmaz
KEYWORDS:
Repaglinide, Metformin, Derivative Spectrophotometry, HPLC, Drug Analysis
JOURNAL NAME:
American Journal of Analytical Chemistry,
Vol.8 No.9,
September
6,
2017
ABSTRACT: In this study, a derivative spectrophotometric
method and one HPLC method were developed
and validated for analysis of anti-diabetic drugs, repaglinide (RPG) and
metformine hydrochloride (MTF) in tablets. The spectrophotometric methods were based on zero-crossing first-derivative and fourth-derivative
spectrophotometric method for simultaneous analysis of RPG (308 nm) and MTF
(267 nm), respectively. Linear relationship between the absorbance at λmax and the drug
concentration was found to be in the ranges of 5.0 - 50.0 μg·mL-1 for both RPG and MTF. The quantification limits for
RPG and MTF were found to be 0.568 and 1.156 μg·mL-1,
respectively. The detection limits were 0.170 and 0.347 μg·mL-1 for
RPG and MTF, respectively. The second method is a rapid stability-indicating
isocratic HPLC method developed for the determination of RPG and MTF. A linear
response was observed within the concentration range of 5.0 - 50.0 μg·mL-1 for both RPG and MTF. The quantification limits for
RPG and MTF were found to be 1.821 and 1.653 μg·mL-1,
respectively. The detection limits were 0.601 and 0.545 μg·mL-1 for
RPG and MTF, respectively. The proposed methods were successfully applied
to the tablet analysis with good accuracy and precision.