TITLE:
Low-Flow Polysulfone Hemodialysis Alters Lipoprotein Parameters, Paraoxonase Activity and in Vitro Incorporation of Phospholipids
AUTHORS:
Franciane S. Marques, Alysson Luiz M. Da Silva, Ricardo J. C. Mattoso, Roque Aras, Rogério Jorge B. De Oliveira, Júlio Cézar De Abreu Santos, Fabio D. Couto, Ricardo D. Couto
KEYWORDS:
Hemodialysis, Dyslipidemia, Cardiovascular Risk Bioindices, PLTP, PON-1, HDL-Remodeling, Low-Flow Polysulfone Dialyzing-Membrane
JOURNAL NAME:
Journal of Biophysical Chemistry,
Vol.8 No.3,
August
30,
2017
ABSTRACT: End stage-renal-disease (ESRD) is associated with dyslipidemia and premature
atherosclerosis. The study evaluates the effect of hemodialysis (HD) on
HDL-remodeling between pre- and post-HD. Was conducted a cross-sectional study
with 50 ESRD male patients, undergoing HD at Ana Neri Hospital, Salvador,
Brazil. All individuals were on HD for at least 3 months, into a three sessions protocol for 3 - 4 hours per week, with a polysulfone low-flow basic-dialyzing-membrane
and unfractionated-heparin. HDL Phospholipid-incorporation was measured by 14C-PL-scintillation-counting,
expressed as %14C-PL/mL/hour. Paraoxonase (PON-1) activity was
measured by spectrophotometry using paraoxon as substrate. Cardiovascular risk
ratios and atherogenic index of plasma were calculated. Total cholesterol, HDL-C and non-HDL-C increased at
post-HD on all age groups, but without triglycerides (TG) changes. TG/HDL-C decreased
in 30 - 39 and 40 - 49 year (y) at post-HD (p 0.05). LDL-C/apoB
increased in >60 y, after HD (p - 49 y (p 0.05) and >60 (p 0.01). On the other hand,
non-HDL-C/HDL-C reduced in 40 - 49 and >60 y, at post-HD (p 0.05). The
linear-correlation between %14C-PL-incorporation and non-HDL-C/HDL-C
was negative in 30 - 39 y, both
at pre-HD (r = -0.90; p = 0.002) and post-HD (r = -0.78; p = 0.022).
Linear-correlation between PON-1 and %14C-PL-incorporation was positive in >60 y, both at pre- (r = 0.63; p = 0.029) and post-HD (r = 0.65; p = 0.022). PON-1 activity increased at pre- (59 ± 30) and post-HD (73 ± 38) in
50 - 59 y (p 0.05). The %14C-PL-incorporation
was reduced in >60 y (p 0.05), when compared to pre-
and post-HD. ESRD
patients undergoing HD shows important changes on lipid-profile, PON-1-activity,
cardiac risk ratios and HDL-remodeling. These results demonstrate the influence
of HD with a polysulfone low-flow basic-dialyzing-membrane and unfractionated-heparin
on lipoprotein metabolism.