TITLE:
Study of the Production of Lentinus crinitus (L.) Fr. Lignolytic Enzymes Grown on Agro-Industrial Waste
AUTHORS:
Taiana de Araújo Conceição, Maria Gabriela Bello Koblitz, Hélio Mitoshi Kamida, Aristóteles Góes-Neto
KEYWORDS:
Lignolytic Enzymes; Lentinus, Agro Waste
JOURNAL NAME:
Advances in Bioscience and Biotechnology,
Vol.8 No.8,
August
22,
2017
ABSTRACT: Exclusively Brazilian, the Caatinga biome has been
thus far a place of very few studies on the Basidiomycetes fungi.
Due to its semiarid climate, fungi found in the region are likely to carry lignolytic enzymes which hold biotechnological potential to be used in industrial
processes of agro-industrial residue bioconversion.
This study performed a response surface statistical planning to optimize
the secretion of enzymes such as laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP) by Lentinus crinitus. Three variables were under analysis: different
concentrations of barley and cassava residue, pH and temperature. MnP
enzyme showed the highest enzymatic activity rate (23.5 IU/L). Additionally, MnP had the best results of enzyme
secretion for substrate composition
of 50% barley and 50% cassava, at pH 7 and temperature at 28°C for a 28-day incubation period. However, further studies are pivotal to test the
efficiency in lignin bioconversion by the
enzymes synthesized in this work and also to establish their usage pattern
on a large scale.