TITLE:
Markers of Heart, Lung and Dorsal Aorta Damage of Mother Rats and Their Neonates Post Therapeutic Treatment with Doxorubicin, Cisplatin and 5-Flurouracil
AUTHORS:
Heba A. El-Ghawet, Abdelalim A. Gadallah, Ahmed A. El-Mansi, Ali H. Amin, Hassan I. H. El-Sayyad
KEYWORDS:
Anticancer Drugs, Heart, Lung, Dorsal Aorta, Isoenzyme Electrophoresis, Biochemical Markers, Amino Acids, DNA Damage
JOURNAL NAME:
Chinese Medicine,
Vol.8 No.3,
August
15,
2017
ABSTRACT: Aim: Recently, there is an increased average of developing cancers. Though,
the chemotherapeutic-treatment is unfavorable during pregnancy due to its
harmful effects on developing fetuses, physicians have two ways to minimize
these effects either by termination of the pregnancy or minimizing its side effects.
The present work aimed to illustrate the susceptibility of cardiac, lung and
dorsal aorta function to the widely applicable drugs doxorubicin and cisplatin
as well as 5-flurouracil. Materials and Methods: Mother albino rats were
arranged into four-groups (control, doxorubicin, cisplatin and 5-flurouracil-treated
groups). Each pregnant rat received intraperitoneal administration of 0.2 mg/kg
body weight at 10th and 14th day of gestation and
sacrificed at parturition (two doses). At parturition, serum of mother rats
used to assess troponin I, heat shock protein 70, 8-hydroxydeoxyguanosine,
vascular endothelial growth factor and adhesion molecules (ICAM-1 & VCAM-1).
Isoenzyme electrophoresis of alkaline and acid phosphatases, glucose-6-phosphate
dehydrogenase and lactic dehydrogenase were estimated in serum, myocardium and
dorsal aorta of mother rats. The myocardium and lung were processed for histopathological
investigations for both mothers and their offspring. Single strand (comet
assay) and double strand DNA damage were carried out in heart and dorsal aorta
of mother rats. Results: The present finding revealed that there are
detected alterations of myocardial markers and lung amino acid metabolism as
well as disruption of myocardial isoenzymes. DNA damage of myocardium and
dorsal aorta were observed. Conclusions: The authors concluded that the
metabolic activity of heart and lung is highly susceptible to doxorubicin and
cisplatin treatment compared to 5-flurouracil and the therapeutic doses must be
degraded.