TITLE:
Two Molecular Markers of Early Non-Small Cell Lung Carcinoma Based on Gene Expression in Peripheral Blood
AUTHORS:
Naghmeh Bahrami, Hamidreza Jamaati, Jalal Heshmat Nia, Payam Tabarsi, Atefeh Fakharian, Habib Emami, Somayeh Sharifynia, Masoum Abniki, Abdolreza Mohamadnia
KEYWORDS:
MUC1 mRNA, CEA mRNA, Non-Small Cell Lung Cancer, Biomarker
JOURNAL NAME:
Open Journal of Clinical Diagnostics,
Vol.7 No.3,
August
14,
2017
ABSTRACT: Background: Lung cancer is among the most common cancers. Search
is ongoing to find biomarkers to improve the diagnosis lung cancer techniques
in early stages. In this study we evaluate the sensitivity and specificity of
the MUC1 and CEA gene expressions in the peripheral blood of non-small cell
lung cancer (NSCLC). Material and Methods: This study was done in Masih
Daneshvari Hospital, Tehran, Iran and was case/control
study that conducted on 30 NSCLC patients and 30 healthy controls. Peripheral
blood was collected and total RNA was extracted then cDNA was synthesized. Sample
was separately assessed by real time PCR. Results: The expression of CEA
gen was positive in 24 patients indicating 80% sensitivity for this marker. The
expression of CEA gen was positive in 9 controls out of 30 each. A
statistically significant difference was detected between patients and healthy
controls with regard to CEA mRNA expression (P 0.001).
The MUC1 gen expressed in 20 out of 30 patients, while it expressed in 3
controls. The difference in MUC1 mRNA expression was statistically significant
between NSCLC patients and healthy controls
(P 0.001). Conclusion: MUC1 and CEA are
molecular biomarkers with relatively favorable sensitivity for primary
diagnosis of NSCLC.