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Sato, S., Jung, H., Nakagawa, T., Pawlosky, R., Takeshima, T., Lee, W.R., Sakiyama, H., Laxman, S., Wynn, R.M., Tu, B.P., MacMillan, J.B., De Brabander, J.K., Veech, R.L. and Uyeda, K. (2016) Metabolite Regulation of Nuclear Localization of Carbohydrate-Response Element-binding Protein (ChREBP): Role of Amp as An Allosteric Inhibitor. The Journal of Biological Chemistry, 291, 10515-10527.
https://doi.org/10.1074/jbc.M115.708982

has been cited by the following article:

• TITLE: Structural Basis for the Interaction of 14-3-3β with Tricarboxylic Acid Cycle Intermediate Malate

  AUTHORS: Zhiqiang Hou, Lijing Su, Xiaoyan Liu

  KEYWORDS: Crystal Structure, 14-3-3 Protein, ChREBP, Transcription Activation, Malate, Malic Acid

  JOURNAL NAME: Journal of Biosciences and Medicines, Vol.5 No.8, August 3, 2017

  ABSTRACT: The protein family of 14-3-3(s) has risen to a position of higher importance as an adaptor protein in cell biology. The seven highly conserved human 14-3-3 proteins coordinate diverse cellular processes including apoptosis, DNA damage response, protein trafficking, and others. In liver hepatocytes, 14-3-3β binds to Ser196-phosphorilated glucose-responsive carbohydrate response element-binding protein (ChREBP) to inhibit converting excess carbohydrate to fat by regulating the nuclear/cytosol trafficking of ChREBP. Here, we report X-ray crystal structures of homodimeric mammalian 14-3-3β in its apo, Malate-bound forms. The determined apo structure was captured with one monomer in the closed state, whereas the other one had an open conformation. Strikingly, 14-3-3β binds Malate dynamically with a double-closed state, which is distinct from all previously characterized 14-3-3(s) and target ligand-binding modes. Malate docks into a first-time observed cofactor pocket located at the concaved interface of 14-3-3β helices α2, α3, α4 through mainly electrostatic and hydrogen interactions. Such a Tricarboxylic Acid Cycle intermediate Malate bond model might offer a new approach to further analyze insulin-independent 14-3-3/ChREBP pathway of de novo fat synthesis in the liver.