TITLE:
Evaluation of Cytotoxicity of Gossypium barbadense L. Extract in Bone Cord Cells through the Micronucleus Test
AUTHORS:
Thiago Dornelas de Oliveira, Joice Meire Rodrigues, Thiago Sande Miguel, Nayrton Kalys Cruz dos Anjos, Juscélio Clemente de Abreu, Lamara Laguardia Valente Rocha, Raquel Xavier Ligeiro Dias, Daniel Almeida da Costa
KEYWORDS:
Gossypium, Mutagenesis, Phytotherapy
JOURNAL NAME:
Journal of Biosciences and Medicines,
Vol.5 No.7,
July
28,
2017
ABSTRACT: The cotton plant is a plant belonging to the family Malvaceae and its leaves are often used in folk medicine. The present study aimed to evaluate the mutagenic or antimutagenic activity of the ethanolic extract of Gossypium barbadense L. (EEG) and to analyze its capacity to prevent or repair mutagenic lesions caused by cyclophosphamide (CP). For the Micronucleus test, Swiss mice were divided into 5 groups: negative control; Positive control: treated with 50 mg/kg cyclophosphamide (CP), group receiving EEG 500 mg/kg for 7 days; Group receiving EEG 500 mg/kg for 7 days associated with CP24h before euthanasia; Group who received CP and EEG 500 mg/kg for 7 days. After the treatment period, the animals were euthanized, the bone marrow removed and the blood smear prepared. From this analysis, it was observed that the EEG caused a significant increase in the number of micronucleated erythrocytes, indicating mutagenic activity of the extract. In addition, it was verified that the extract did not present the capacity to prevent, but it presented the capacity of repair of the chromosomal damages caused by CP.