TITLE:
Response Surface Optimization of a Solvent Free Production of cis-9, trans-11 and trans-10, cis-12 Isomers of Conjugated Linoleic Acid Using High Linoleic Sunflower Oil
AUTHORS:
Sara Koohikamali
KEYWORDS:
CLA Methyl Esters, Isomerization, Commercial CLA, RSM, Phase Transfer Catalyst, PEG400
JOURNAL NAME:
Food and Nutrition Sciences,
Vol.8 No.6,
June
28,
2017
ABSTRACT: The
most dominant and beneficial conjugated linoleic acids isomers (CLAs) with
miscellaneous biological tasks are 9c11t-C18:2 and 10t12c-C18:2. The problem
with most of the commercial CLA produced by non-optimized conventional
approaches is the heterogeneity of their isomers and undesirable toxic
by-products. In this study, optimization of the isomerization of the fatty acid
methyl esters of the high linoleic sunflower oil was investigated through
response surface methodology (RSM). The reaction temperature, the concentration
of PEG400 and NaOCH3 had positive influence on the total conjugated
linoleic acid methyl esters (CLAME) production as response. However, the effect
of the polyethylene glycol 400 (PEG400) concentration was more significant on
response than those of other factors (p i.e., based on the mass percentage of total fatty acid methyl
esters mixture) was at 5% w/w NaOCH3, 1.06% w/w PEG400 and
temperature of 140°C.