TITLE:
Antimicrobial Activity of Wild Plant Seed Extracts against Human Bacterial and Plant Fungal Pathogens
AUTHORS:
Valentina I. Pushkareva, Marina P. Slezina, Tatyana V. Korostyleva, Larisa A. Shcherbakova, Ekaterina A. Istomina, Svetlana A. Ermolaeva, Olga A. Ogarkova, Tatyana I. Odintsova
KEYWORDS:
Plant Extracts, Antibacterial Activity, Human Food-Borne Pathogens, Antifungal Activity, Plant Pathogenic Fungi
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.7,
June
21,
2017
ABSTRACT: Five wild plant species belonging to different families
(Chenopodium album, Plantago major, Elytrigia elongata, Filipendula ulmaria and Nigella sativa) widely spread in Russian
Federation and the former USSR were evaluated for their ability to inhibit growth
of two important human food-borne pathogens (Escherichia coli O157:H7
and Listeria monocytogenes strain
EGD-e) and eight plant pathogens (Alternaria alternata, Alternaria tenuissima, Bipolaris sorokiniana, Stagonospora nodorum, Fusarium solani, Fusarium oxysporum, Fusarium culmorum and Phytophtora infestans). To isolate biologically
active compounds from seeds, a step-wise procedure including extraction with hexane,
ethyl acetate, ethanol, and 10% acetic acid followed by reversed-phase HPLC was
developed. Using disc-diffusion assay, the highest activity against E. coli O157:H7 was observed with
extracts from F. ulmaria (hexane
and ethyl acetate extracts and the unbound RP-HPLC fraction) and P. major (ethyl acetate extract and
the unbound RP-HPLC fraction); E. elongate (the unbound
RP-HPLC fraction) was less active. The extracts from P. major and E. elongate (the unbound RP-HPLC fractions)
were equally highly active against L. monocytogenes, while
those of F. ulmaria (the unbound
RP-HPLC fraction) and N. sativa (hexane
and ethyl acetate extracts) were less active against this pathogen. The dynamics
of L. monocytogenes EGD-е and E. coli O157:H7
growth in the presence of two most potent extracts (RP-HPLC-unbound fractions of P. major and E. elongate and the hexane extract of F. ulmaria) was studied.
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