TITLE:
Chemical Composition, Antioxidant and Antimicrobial Activity of the Oil and Plant Extract Myrocarpus frondosus Allemão
AUTHORS:
Ivandra Ignês de Santi, Darci Alberto Gatto, Miriam Ribeiro Galvão Machado, Patrícia Soares Bilhalva dos Santos, Rogerio Antônio Freitag
KEYWORDS:
Myrocarpus frondosus Allemão, Antimicrobial, Antioxidant
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.7,
June
16,
2017
ABSTRACT: The
objective of this study was to evaluate the chemical profile of extracts
(aqueous and ethanol) and the essential oil of Myrocarpus frondosus Allem?o, the
sensitivity of strains of Escherichia coli, Staphylococcus
aureus, Salmonella
typhimurium and Listéria monocytogenes, front
the extracts and essential oil, by means of the microdilution method in broth,
the antioxidant activity by the ABTS method, and the content of phenolic compounds
present in the extracts and oil. For the preparation of extracts from plant
leaves used with ethanol and water, then separated, the chemical identification
of compounds was performed by high-performance liquid chromatography (CLAE-DAD)
and gas chromatography coupled to mass spectrum (CG/MS). With the chemical
analysis of the extracts obtained the presence of the major compound rutin, and
oil major compound was found germacrene B. In the microdilution method in
broth, oil and extracts showed inhibition against all the bacteria tested in
the concentrations 1 mg/ml
to 0.25 mg/ml, except for Staphylococcus aureus at a concentration of
0.25 mg/ml of the essential oil and trans-caryophyllene.
The results of Minimum Bactericidal Concentration showed that the essential oil had bactericidal activity at a
concentration of 1mg/ml for all bacteria tested and trans-caryophyllene at the
same concentration only for Listeria monocytogenes. In
relation to the essential oil, antioxidant activity showed
higher radical reduction capacity of 40.92% and the content of phenolic compounds
ethanol extract showed more 12.72%. The in vitro results support the
conclusion that the essential oil is very promising both in antimicrobial
action as antioxidant activity and the leaf extracts on antioxidant activity.