TITLE:
Expression Vector Construction and Genetic Transformation of Paeonia lactiflora Gibberellin 20-Oxidase Gene
AUTHORS:
Lulu Han, Junjie Li, Jing Guo, Yan Ma, Xianfeng Guo
KEYWORDS:
PlGA20ox, Expression Vector, Arabidopsis thaliana, Transformation
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.7,
June
16,
2017
ABSTRACT: GA20-oxidase (GA20ox) gene encodes a key
enzyme in gibberellins (GAs) biosynthesis pathway. Previously, we have cloned a PlGA20ox gene (GeneBank accession
number: KU886552) from Paeonia lactiflora. To further reveal
its function, we constructed an expression vector in the present study and then
transformed it into Arabidopsis thaliana plants by floral dip method. The transgenic plants
exhibited an early bolting, increased height and improved vegetative growth.
These results provided efficient vector tool and functional information of PlGA20ox for future gene
engineering in peony.