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Guo, H.L., Zhou, X.H., Zhang, Y., Song, B.D., Liu, L.H., Zhang, J.X. and Shi, H.C. (2014) Highly Sensitive and Rapid Detection of Melamine in Milk Products by Planar Waveguide Fluorescence Immu-nosensor (PWFI). Sensors and Actuators B, 194, 114–119. https://doi.org/10.1016/j.snb.2013.12.058

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• TITLE: A Portable Evanescent Wave Optic Fiber Immunosensor for Sensitive and Rapid Detection of 2,4-D in Water Samples

  AUTHORS: Wei Li, Jun Wu, Rong Yang, Dan Song, Feng Long

  KEYWORDS: Evanescent Wave Optic Fiber Immunosensor, 2, 4-D, Detection, Immunoassay

  JOURNAL NAME: Journal of Geoscience and Environment Protection, Vol.5 No.6, June 12, 2017

  ABSTRACT: A portable evanescent wave optic ?ber immunosensor was developed for detection of 2,4-dichlorophenoxyacetic acid(2,4-D) using a indirect competitive immunoassay. In this paper, hapten conjugates 2,4-D-OVA were immobilized with covalent binding methods. After pre-reacting, 2,4-D-antibody-Cy5.5 in sample specifically recognized the 2,4-D antigens binding sites on surface of the optical fiber probe. Under optimum conditions, 2,4-D could be detected in less than 18 min for each assay cycle. The regeneration of the optic ?ber surface allowed more than 200 times without losing performance. The limits of detection of 0.039 ug/L and the quantitative detection range of 0.47 - 81.02 ug/L were obtained when the concentration of 2,4-D was 1 mg/L. This immunosensor shows great potential in rapid simultaneous detection of 2,4-D in waters samples.