TITLE:
Developing Stable Cultivar through Microspore Mutagenesis in ×Brassicoraphanus koranhort, Inter-Generic Allopolyploid between Brassica rapa and Raphanus sativus
AUTHORS:
Soo-Seong Lee, Byoung Ho Hwang, Tae Yoon Kim, Jeongmin Yang, Na Rae Han, Jongkee Kim, Hyun Hee Kim, Hadassah Roa Belandres
KEYWORDS:
xBrassicoraphanus, Intergeneric Hybrid, Baemoochae, Brassica rapa, Raphanus sativus
JOURNAL NAME:
American Journal of Plant Sciences,
Vol.8 No.6,
May
26,
2017
ABSTRACT: A stable progeny was developed
through induced mutation, using microspore culture, of the hybrid (F1F1)
produced by crossing a newly synthesized, unstable allopolyploid (F1)
and a stable cultivar, BB#1(F1)
in xBrassicoraphanus. An F1F1 plant
was subjected to the induced mutation system established during production of BB#1.
Morphological characteristics of the progeny such as color, and leaf number and
length, differed from those of BB#1. The bolting time of the progeny in spring cropping
was very late compared to BB#1, allowing it to be grown to an adult plant in spring.
Genomic in situ hybridization analysis of pollen
mother cells at prophase I identified 19 bivalents, 10 from Brassica rapa and 9 from Raphanus sativus. The glucoraphenin content was
almost identical to that of BB#1. Two cultivars are available in the baemoochae
crop now. These results indicate that induced mutation using microspore culture
is a viable method of stabilizing intergeneric allopolyploids between B. rapa and R. sativus.