TITLE:
Symbiotic, Hypocholesterolemic and Antioxidant Effects of Potential Probiotic Lactobacilli Strains Isolated from Tunisian Camel Milk
AUTHORS:
Imen Mahmoudi, Olfa Ben Moussa, Mnasser Hassouna
KEYWORDS:
Probiotic, Prebiotic, Cholesterol Removal, Bile Salt Deconjugation, Antioxidant Activity, Functional Food
JOURNAL NAME:
Advances in Microbiology,
Vol.7 No.4,
April
30,
2017
ABSTRACT: In the present study, 20 selected Lactobacillus strains already characterized in a previous
research for their capability to grow in conditions simulating the intestinal
environment, their resistance to antibiotics, their antibacterial activity and
their adhesion capability to intestinal human Caco-2 TC7 and HT-29 MTX cell
lines, were further investigated to explore more their probiotic properties.
Growth behaviour in the presence of prebiotic (fructooligosac-charides (FOS) and lactulose) at a concentration of 2%, cholesterol
removal by measuring the residual cholesterol in medium supplemented with cholesterol, ability to deconjugate bile salts
using BSH enzyme and
antioxidant activity of culture supernatant
of Lactobacillus strains by ABTS·+ and DPPH methods were analyzed. All probiotic strains demonstrated
important prebiotic assimilation (P > 0.05) even with OD600 > 3 after 30 h of contact, cholesterol removal ability with maximum
percentage of 57% after 24 h of contact and they were found to liberate
significantly (P ·+ and DPPH respectively. This study confirmed the suitability of these
probiotic strains for application in functional food formulations especially
where cholesterol reduction and antioxidant activity in food are needed to
assess possible in vivo human health benefits.