TITLE:
New Amphiphilic Amino Acid Derivatives for Efficient DNA Transfection in Vitro
AUTHORS:
Lucía C. Peña, María F. Argarañá, María M. De Zan, Antonella Giorello, Sebastián Antuña, Claudio C. Prieto, Carolina M. I. Veaute, Diana M. Müller
KEYWORDS:
Amphiphile, N-Acylated, Cysteine, Gemini, Ornithine, Transfection
JOURNAL NAME:
Advances in Chemical Engineering and Science,
Vol.7 No.2,
April
11,
2017
ABSTRACT: Nucleic acids-based therapies have recently developed as next-generation agents for treating and preventing viral infection, cancer, and genetic disorders, but their use is still limited due to its relatively poor delivery into targeted cells. We designed and synthesized new amphiphilic amino acid derivatives (cysteine-based) of low molecular weight, formed by the same pentapeptide (AG2: WWCOO) N-acylated, with different hydrophobic chains containing from 12 to 18 carbons, named AG2-Cn (N), which dimerize by oxidation in the presence of pLenti-CMV-GFP Puro plasmid (P) in the respective gemini. We determined transfection efficiency, critical micelle concentration, particle size, ζ-potential and cytotoxicity for the derivatives obtained. We found that all the synthesized compounds were active for DNA delivery and had greater ability to transfect CHO-K1 cells. In particular, AG2-C18 is a promising carrier for gene delivery because it showed no cytotoxicity and its activity was greater than or equal to the commercial actives currently used.