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Britto, P., Lye, S., Proulx, K., Yousafzal, A.K., Matthews, S., Viavada, T., Perez, R., Rao, N., Ip, P., Fernald, L.C., MacMillan, H., Hanson, M., Wachs, T.D., Yao, H., Yoshikawa, H., Cerezo, A., Leckman, J.F. and Bhutta, Z. (2017) Nurturing Care Promoting Early Childhood Development. The Lancet, 389, 91-102. https://doi.org/10.1016/S0140-6736(16)31390-3

has been cited by the following article:

• TITLE: Fetal RBCs Potential in Obstetric Protocols to Minimize Fetomaternal Hemorrhage

  AUTHORS: Hasnaa A. Abo-Elwafa, Salah A. Ismail, Ibrahim M. A. Hassanin, Rania M. Ahmed

  KEYWORDS: Fetomaternal Hemorrhage, Obstetric Protocols, Flow-Cytometer

  JOURNAL NAME: Open Journal of Blood Diseases, Vol.7 No.1, March 31, 2017

  ABSTRACT: Background: During pregnancy a small number of fetal RBCs (Red Blood Cells) enter maternal blood without risk, and incidence of significant feto-maternal hemorrhage (FMH) and isoimmunization depends on how much fetal blood enters maternal circulation; the use of flow cytometer (FCM) in FMH detection is considered the best laboratory technique. Aims: To evaluate role of FCM in FMH estimation to optimize delivery protocols and decrease isoimmunization. Subject and Method: 100 pregnant women at labor were included, equally classified into early cord clamping (ECC) and delayed cord clamping (DCC) groups, each including 25 women delivered vaginally and 25 with caesarian section; the control groups included 20 non-pregnant females, 20 at 3rd trimester and 20 neonates. Fetal RBCs were done on Becton Dickinson (B.D) FCM, and the reagents used were 5-Glutaraldehyde 0.5% Sigma cat. G5882, flouroscine isothiocyanite (FITC) anti-hemoglobin F (Hb-F) cat. 555748, anti-carbonic anhydrase, Triton-X100 solution and Alsever’s solution cat. HFH-11, 0.1% phosphate buffer saline/bovine serum albumin (PBS/BSA). Statistical Analysis: Mann Whitney’s U-test, Chi-squared, Fischer’s Exact tests, and SPSS for windows version 15.0. Results: There was a significant increase in the volume of the FMH in the ECC compared to DCC groups (p