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Von Haefen, C., Wendt, J., Semini, G., Sifringer, M., Belka, C., Radetzki, S., Reutter, W., Daniel, P.T. and Danker, K. (2011) Synthetic Glycosidated Phospholipids Induce Apoptosis through Activation of FADD, Caspase-8 and the Mitochondrial Death Pathway. Apoptosis, 16, 636-651.
https://doi.org/10.1007/s10495-011-0592-2

has been cited by the following article:

• TITLE: OSW-1 Induced Apoptosis in Hepatocellular Carcinoma through Generation of ROS, Cytochrome C and Noxa Activation Independent of p53 with Non-Activation of Caspase-3

  AUTHORS: Xiaochen Liu, Jingchao Liang, Jichun Jin, Haiyang Li, Bosheng Mei, Xinglin Jin

  KEYWORDS: OSW-1, Hepatocellular Carcinomas, ROS, Cytochrome C, Mitochondrial Death Pathway

  JOURNAL NAME: Chinese Medicine, Vol.8 No.1, March 14, 2017

  ABSTRACT: Aim: To study the antitumor mechanism of OSW-1 in hepatocellular carcinoma. Materials and Methods: The expression profiling microarray was carried out to extract RNA from SK-Hep-1 which suffered from OSW-1. ρ0-SK-Hep-1 was maintained SK-Hep-1 in MEM containing 100 μg/L ethidium bromide (EB), 1 mM sodium pyruvate and 50 μg/ml uridine for 40 days. Then confirmed COX-I and COX-II of mitochondrial DNA were knocked out. Cells suffered from OSW-1 or doxorubicin. Then cells were washed twice with cold PBS and incubated with DCFH-DA. Fluorescent signal was recorded by using Infinite 200 Pro multimode Plate readers. Results: OSW-1 elevates generation of ROS and Cytochrome C which are associated with the induction of apoptosis in SK-Hep-1 cells. We also demonstrate that OSW-1 does not depend on p53 to up-regulate the BH3-only protein Noxa. What is more noteworthy that the Caspase-9 and FADD are down-regulated in above process. Conclusion: OSW-1 induced special apoptosis is different from the mitochondrial death pathway and the death receptor pathway and final result is not Caspase family’s activating. This provides a novel theory that nonmalignant cells are significantly less sensitive to OSW-1 than cancer cell lines.